Session:

Physiological and pathological processes within the human body are controlled by complex cell-cell interactions within the context of a dynamic microenvironment. The ability to dynamically measure phenotypes (i.e. gene expression, protein activities, ion fluctuations, signaling) at the single cell level is key to understanding cellular behaviour in a complex environment.
I will present the integration of nanopipettes with scanning probe microscopy techniques to enable the extraction of genetic material from living cells. I will discuss the longitudinal profiling of brain cancer cells to characterize the transcriptional reprogramming at the single cell level after chemotherapy and radiotherapy.

Co-Author(s): Fabio Marcuccio (University Of Leeds), Chalmers Chau (University Of Leeds), Lucy Stead (University Of Leeds)

Paolo Actis

University of Leeds

Appearances