To view this presentation:

  • If you have an MRS account, click the Login button above.
  • New to MRS?  Create a free account here



  0       0

2014 MRS Spring Meeting


Z10.02 - Analysis of Epithelial Cell Barrier Function in a Three Dimensional Culture Using an Organic Electrochemical Transistor


Apr 24, 2014 2:00pm ‐ Apr 24, 2014 2:15pm

Description

Cellular lines models are widely used to study cell behavior under different conditions. Epithelial cells are the most analyzed in vitro system, especially when they are grown as monolayers over a solid surface in two dimensional cultures. In these conditions, the cells keep many of the original characteristics; however recent reports show that the use of solid matrix to growth cells, or three dimensional (3D) cultures, mimics tissue characteristics, so the response to a specific stimulus is different than in monolayer, and more in vivo like.

One of the protein complexes dedicated to sense these changes is the tight junction (TJ), which is localized in the most apical region of the epithelial cells. The main technique to analyze the barrier function of tight junction is the measurement of the transepithelial electrical resistance, which determines the ion flux through TJ; previously in our group an Organic Electrochemical Transistor (OECTs) was developed to accurately record the TJ barrier activity. In the present work we combine the 3D culture and the OECTs to analyze the tight junction in a 3D system such as kidney epithelial cyst. We determined the optimum conditions of the cyst culture in a Matrigel matrix, the biochemical characterization, as well as the barrier properties of the tight junctions under different stimuli.

Speaker(s):

You must be logged in and own this session in order to post comments.

Print Certificate
Review Answers
Print Transcript
Completed on: token-completed_on
Review Answers
Please select the appropriate credit type:
/
test_id: 
credits: 
completed on: 
rendered in: 
* - Indicates answer is required.
token-content

token-speaker-name
token-index
token-content
token-index
token-content
token-index
token-content
token-index
token-content
token-index
token-content
token-index
token-content
/
/
token-index
token-content
token-index
token-content