Label-free detection based on optical techniques has revolutionized the ability to detect a broad range of biological samples such as protein-protein interaction, DNA hybridization and cell membrane dynamics simply based on the intrinsic dielectric permittivity of samples without the need for labeling. In particular, label-free optical techniques such as surface plasmon resonance, photonic crystal, and ring resonator have been integrated with microfluidics to measure the changes in the refractive index near the surface of the sensor. However, these techniques require complex instrumentation for illumination and detection such as high-resolution spectrophotometer, high-intensity monochromatic light source, and prism coupling. In contrast, optical sensors consisted of nanohole array on a noble-metal film, which exhibit extraordinary optical transmission (EOT), can be used with similar sensitivity to detect the changes in the refractive index near the surface of the sensor, with the advantage of simple collinear broadband illumination and portable spectrophotometer. Previously, we have reported colorimetric surface plasmon resonance imaging using a nanohole array device called nanoLycurgus Cup Array (nanoLCA), in which we demonstrated high spectral sensitivity of the nanoLCA to the changes in the refractive index due to the presence of different refractive index solutions and to the surface binding of biological-relevant molecules such as protein-protein interaction and DNA hybridization. Due to the unique transmission/reflection peak wavelengths of nanoLCA in the visible wavelength range, we were able to demonstrate visible colorimetric changes simply due to the changes in the refractive index on and near the surface of the sensor upon the changes of the presence or the concentration of the sample of interest. In this work, we extended the capability of nanoLCA to on-chip applications by integrating relevant microfluidic and micro-well plate designs, such as parallel flow channels, droplet generator, and by demonstrating colorimetric visualization of static and transient dynamics of optically-transparent solutions, cell membrane dynamics and molecular binding events which previously could not be visualized in a colorimetric manner using ubiquitous laboratory equipment such as bright field microscope and plate reader.